Integrated attributes

No	Attribute	Description
1	Age	Age at the time of sampling; relevant scale depends on species and study, e.g. could be seconds for amoebae or centuries for trees. For samples such as cell lines in which age is not applicable, enter not applicable. For samples where an exact age is unknown, such as when only the range of age values is known, enter missing:not collected in this field, then use the “other characteristics“ field to enter the range of age values Example: 28 years, 2.5 months, 13 weeks, 3 days, 10 embryo age
2	Altitude	The altitude of the sample is the vertical distance between Earth's surface above sea level and the sampled position in the air Example: 1,000 m
3	Biomaterial Provider	Name and address of the lab or PI, or a culture collection identifierr. If a value cannot be provided, submitters are asked to use one of the accepted missing value reporting terms Example: Korean Collection for Type Cultures(KCTC), Korean Cell Line Bank(KCBL), Dr. Gildong Hong from Seoul National University
4	Birth date	Birth date of the subject Format: YYYY-MM-DD, YYYY-MM, YYYY Example: 1990-10-30, 1990-10, 1990
5	Birth location	Birth location of the subject Example: Laboratory Animal Resource & Research Center - KRIBB (LARRC), Johns Hopkins Hospital Gynecology & Obstetrics
6	Breed	Breed name - chiefly used in domesticated animals or plants Example: BALB/c, C57BL/6, White (w1118)
7	Breeding history	Breeding history
8	Breeding method	Breeding method
9	Broad-scale environmental context	Add terms that identify the major environment type(s) where your sample was collected. Recommend subclasses of biome [ENVO:00000428]. Multiple terms can be separated by one or more pipes (|) Format: http://purl.obolibrary.org/obo/ENVO_00000428 or ENVO_00000428 in EBI Ontologies Example: mangrove biome [ENVO:01000181]|estuarine biome [ENVO:01000020]
10	Cell line	Name of the cell line
11	Cell subtype	Subtype of a cell
12	Cell type	Type of cell of the sample or from which the sample was obtained
13	Collected by	Name of persons or institute who collected the sample
14	Collection date	The date (or date and time) on which the sample was collected. If a value of an expected format cannot be provided, submitters are asked to use one of the accepted missing value reporting terms. Format: Use ISO 8601 standard on date and time. For date, use the formats “YYYY”, “YYYY-MM”, “YYYY-MM-DD”. For date and time, use the format “YYYY-mm-ddThh:mm:ssZ”. In this format, time is in Coordinated Universal Time (UTC), otherwise known as Zulu time, the letter “T” is added between date and time, and the letter “Z” (indicating Zulu) is added after time. For a range of two date and time values, use the forward-slash character “/” as the delimiter Example: 1990-10-30, 1990-10, 1990, 1952-10-21/1953-02-15, 2015-10-11T17:53:03Z
15	Cultivar	Cultivar name - cultivated variety of plant
16	Culture collection	Name of source institute and unique culture identifier Format: See https://www.insdc.org/submitting-standards/controlled-vocabulary-culturecollection-qualifier/ for the description for the proper format and list of allowed institutes
17	Death date	Death date of the subject Format: YYYY-MM-DD, YYYY-MM, YYYY Example: 1990-10-30, 1990-10, 1990
18	Depth	Depth is defined as the vertical distance below surface, e.g. for sediment or soil samples depth is measured from sediment or soil surface, respectively. Depth can be reported as an interval for subsurface samples Example: 10 m
19	Derived from	Indicates when one BioSample was derived from another BioSample. Value should include BioSample accession number(s) Example: SAMN00000001, KAS24095074
20	Description	Description of the sample
21	Development stage	Developmental stage at the time of sampling Example: adult, embryo, fetal, hatching stage, infant, juvenile, larva, newborn, pluripotency, pupa, R3 growth stage, seedling, vegetative stage
22	Disease	Name of diseases diagnosed Format: Can include multiple diagnoses. Controlled vocabulary from (1) Human Disease Ontology or (2) MeSH
23	Disease stage	Stage of disease at the time of sampling
24	Ecotype	A population within a given species displaying genetically based, phenotypic traits that reflect adaptation to a local habitat Example: Korea, Columbia
25	Ethnicity	Ethnicity of the subject
26	Genotype	Observed genotype
27	Geographic location	Geographical origin of the sample. If a value of an expected format cannot be provided, submitters are asked to use one of the accepted missing value reporting terms. Format: Use the appropriate name from the list shown in https://www.insdc.org/submitting-standards/geo_loc_name-qualifier-vocabulary/. Use a colon (:) to separate the country or ocean from more detailed information about the location Example: “South Korea: Seoul”, “Canada: Vancouver”, “Germany: halfway down Zugspitze, Alps”
28	Growth protocol	Protocol for growth Example: 30 min egg collections of OreR and yw flies at 25 degree Celsius were aged at room temperature (RT) according to the different temporal classes T0-T4.
29	Health state	Health or disease status of sample at time of collection
30	Height or length	Measurement of height or length
31	Host	The natural (as opposed to laboratory) host to the organism from which the sample was obtained. Use the full taxonomic name Example: Homo sapiens
32	Host age	Age of host at the time of sampling
33	Host description	Additional information not included in other defined vocabulary fields
34	Host disease	Name of relevant disease Format: Controlled vocabulary from (1) Human Disease Ontology or (2) MeSH (Medical Subject Heading) Example: Salmonella gastroenteritis
35	Host disease outcome	Final outcome of disease Example: death, chronic disease, recovery
36	Host disease stage	Stage of disease at the time of sampling
37	Host health state	Information regarding health state of the individual sampled at the time of sampling
38	Host sex	Gender or physical sex of the host Controlled Term: "male", "female", "pooled male and female", "neuter", "hermaphrodite", "intersex", "not determined", "missing", "not applicable", "not collected"
39	Host subject id	A unique identifier by which each subject can be referred to, de-identified Example: #131
40	Host tissue sampled	Type of tissue the initial sample was taken from Format: Controlled vocabulary from BRENDA Tissue and Enzyme Source Ontology
41	Identified by	Name of the taxonomist who identified the specimen
42	Isolate	Identification or description of the specific individual from which this sample was obtained
43	Isolation source	Describes the physical, environmental and/or local geographical source of the biological sample from which the sample was derived
44	Karyotype	Karyotype
45	Lab host	Scientific name and description of the laboratory host used to propagate the source organism or material from which the sample was obtained Example: Escherichia coli DH5a, or Homo sapiens HeLa cells
46	Latitude and longitude	The geographical coordinates of the location where the sample was collected. When the information is lacking or specification of location is not appropriate, submitters are asked to use one of the accepted missing value reporting terms. Format: Specify as degrees latitude and longitude in format “d[d.dddd] N|S d[dd.dddd] W|E” Example: 38.98 N 77.11 W
47	Mating type	Mating type of microbe
48	NCBI taxonomy ID	NCBI’s taxonomy identifier of the organism for this sample. The NCBI taxonomy ID can be found at NCBI taxonomy. Enter 32644 (which is a taxonomy ID for unidentified organisms) for the following or similar cases: (1) when NCBI taxonomy ID is not available because NCBI taxonomy does not yet cover the organism, (2) when metagenome or environmental sample was used, whose organismal composition is unknown in advance Example: 9606 for Homo sapiens and 452680 for Pseudomonas sp. UK4
49	Organism	The most descriptive organism name for this sample (to the species, if possible). In the case of a new species, provide the desired organism name. In the case of unidentified species, choose the appropriate Genus and include ‘sp.’, e.g. “Escherichia sp.”. When sequencing a genome from a non-metagenomic source, include a strain or isolate name too, e.g. “Pseudomonas sp. UK4” Example: Homo sapiens, Pseudomonas sp. UK4
50	Passage history	Number of passages and passage method
51	Pathotype	Some bacterial specific pathotypes Example: Escherichia coli - STEC, UPEC
52	Phenotype	Phenotype of sampled organism Format: Controlled vocabulary from Phenotypic Quality Ontology (PATO)
53	Population	Applicable for human and plants. Population is a summation of all the organisms of the same group or species, which live in a particular geographical area, and it can also indicate filial generation, number of progeny, or genetic structure
54	Race	Race of the subject
55	Reference for biomaterial	Primary publication or genome report Format: PubMed ID, or DOI (Digital Object Identifier), or URL
56	Relationship to oxygen	Aerobic or anaerobic Controlled Term: "aerobe", "anaerobe", "facultative", "microaerophilic", "microanaerobe", "obligate aerobe", "obligate anaerobe"
57	Sample collection device or method	Method or device employed for collecting sample
58	Sample material processing	Processing applied to the sample during or after isolation
59	Sample name	A name that you choose for the sample. It can have any format, but we suggest that you make it concise, unique and consistent within your lab, and as informative as possible. Every sample name from a single submitter must be unique within a single BioProject
60	Sample size	Amount or size of sample (volume, mass or area) that was collected
61	Sample type	Sample type, such as cell culture, mixed culture, tissue sample, whole organism, single cell, metagenomic assembly
62	Serotype	Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties Example: serotype=“H1N1” in Influenza A virus CY098518.
63	Serovar	Taxonomy below subspecies; a variety (in bacteria, fungi or virus) usually based on its antigenic properties. Sometimes used as species identifier in bacteria with shaky taxonomy Example: Leptospira, serovar saopaolo S76607 (65357 in Entrez).
64	Sex	Physical sex of sampled organism Controlled Term: "male", "female", "pooled male and female", "neuter", "hermaphrodite", "intersex", "not determined", "missing", "not applicable", "not collected"
65	Source material identifiers	Unique identifier assigned to a material sample used for extracting nucleic acids, and subsequent sequencing. The identifier can refer either to the original material collected or to any derived sub-samples
66	Specimen voucher	Identifier for the physical specimen. When the information is not available or not applicable, submitters are asked to use one of the accepted missing value reporting terms. Format: Use the format “[<institution-code>:[<collection-code>:]]<specimen_id>”, in which collection-code is optional, along with a controlled vocabulary provided at http://www.insdc.org/submitting-standards/controlled-vocabulary-specimenvoucher-qualifier/ Example: KCLB:30082, KCTC:6910, KACC:44679 Note: The institution-code above will be found at https://ftp.ncbi.nlm.nih.gov/pub/taxonomy/coll_dump.txt, an institution-code list file provided by NCBI
67	Storage conditions	Explain how and for how long the soil sample was stored before DNA extraction
68	Strain	Microbial or eukaryotic strain name
69	Stud book number	A number of the captive animal individual in stud book
70	Subgroup	Taxonomy below subspecies; sometimes used in viruses to denote subgroups taken from a single isolate
71	Subtype	Used as classifier in viruses Example: HIV type 1, Group M, Subtype A
72	Temperature	Temperature of the sample at time of sampling Format: The temperature in Celsius is expressed in degrees C, in Fahrenheit in degrees F, and in absolute temperature in K. Please input the temperature value with the corresponding unit (C, F, or K) Example: 37 degrees C, 98.6 degrees F, 310.15 K
73	Tissue	Type of tissue the sample was taken from Example: liver, plasma, urine, leaf, root
74	Treatment	Describes how the sample was treated Example1: Embryos were dechorionated with 50% bleach, put on a cover slip and covered with Halocarbon oil 27 (Sigma). Embryos of the appropriate stage were manually selected under the dissecting scope. Selected embryos were transferred to a basket, rinsed with PBS with 0.7% NaCl, 0.04% triton-X100 and placed on ice in the Trizol solution (GibcoBRL) Example2: The rats were treated with 150 mg phthalate per bodyweight kilogram for each day for 13 weeks.
75	Other characteristics	Other characteristics needed to describe sample characteristics, which can be useful to enter the information on experimental factors. Do not enter this field if there are no other characteristics that are needed to describe the sample Format: This field consists of a pair of two sub-fields: otherCharacteristics_key and otherCharacteristics_value, which correspond to the name of the factor and its value, respectively. Fill out the two sub-fields in a pair. For multiple characteristics, use “;” as a delimiter to enter in the Excel template. Example: BRCA 1 mutation - Yes, BRCA1 mutation status;Chemotherapy dosage - Yes;High, BRCA1 mutation status;Chemotherapy dosage;Weight in kg - Yes;High;65